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Image Search Results
Journal: Journal of the American Society of Nephrology
Article Title: Calcitonin Has a Vasopressin-like Effect on Aquaporin-2 Trafficking and Urinary Concentration
doi: 10.1681/asn.2009121267
Figure Lengend Snippet: Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the epifluorescence microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Article Snippet: Mounted slides were examined using a
Techniques: Electron Microscopy, Clinical Proteomics, Membrane, In Vivo, Labeling, Epifluorescence Microscopy, Software
Journal:
Article Title: T?te ? T?te of Tomato Yellow Leaf Curl Virus and Tomato Yellow Leaf Curl Sardinia Virus in Single Nuclei
doi: 10.1128/JVI.78.19.10715-10723.2004
Figure Lengend Snippet: In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and epifluorescence microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).
Article Snippet: Rehydrated specimens were analyzed with
Techniques: In Situ, Infection, Labeling, Staining, Epifluorescence Microscopy, Microscopy