eclipse e-800 epifluorescence microscope Search Results


99
Nikon eclipse e800 epifluorescence microscope
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Eclipse E800 Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss axiocam hrc digital camera
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Axiocam Hrc Digital Camera, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon e800 epifluorescence microscope 188
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
E800 Epifluorescence Microscope 188, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss epifluorescence microscopes (axioskop
In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and <t>epifluorescence</t> microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).
Epifluorescence Microscopes (Axioskop, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon c1 confocal microscopy attachment
In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and <t>epifluorescence</t> microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).
C1 Confocal Microscopy Attachment, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss lsm710 confocal laser scanning microscope
In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and <t>epifluorescence</t> microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).
Lsm710 Confocal Laser Scanning Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss upright lsm510 confocal microscope
In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and <t>epifluorescence</t> microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).
Upright Lsm510 Confocal Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Zeiss microscope zeiss axioplan 2
In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and <t>epifluorescence</t> microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).
Microscope Zeiss Axioplan 2, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon transmitted epifluorescence light microscope
In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and <t>epifluorescence</t> microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).
Transmitted Epifluorescence Light Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nikon nikon e800 upright microscope
In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and <t>epifluorescence</t> microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).
Nikon E800 Upright Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Systems Inc nikon e800 epifluorescence microscope
In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and <t>epifluorescence</t> microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).
Nikon E800 Epifluorescence Microscope, supplied by Image Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+e-800+epifluorescence+microscope/10__1074_slash_jbc__m408273200-83-8-11?v=Image+Systems+Inc
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Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the epifluorescence microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.

Journal: Journal of the American Society of Nephrology

Article Title: Calcitonin Has a Vasopressin-like Effect on Aquaporin-2 Trafficking and Urinary Concentration

doi: 10.1681/asn.2009121267

Figure Lengend Snippet: Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the epifluorescence microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.

Article Snippet: Mounted slides were examined using a Nikon Eclipse E800 epifluorescence microscope equipped with a 40 1.0NA Plan Apo objective, and the images were captured digitally using a Hamamatsu Orca CCD camera and IPLab Spectrum software (Scanalytic, Vienna, VA).

Techniques: Electron Microscopy, Clinical Proteomics, Membrane, In Vivo, Labeling, Epifluorescence Microscopy, Software

In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and epifluorescence microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).

Journal:

Article Title: T?te ? T?te of Tomato Yellow Leaf Curl Virus and Tomato Yellow Leaf Curl Sardinia Virus in Single Nuclei

doi: 10.1128/JVI.78.19.10715-10723.2004

Figure Lengend Snippet: In situ detection discriminating between TYLCSV and TYLCV in N. benthamiana tissues from stems of doubly infected N. benthamiana, hybridized with a biotin-labeled TYLCSV probe staining red (b) or a DIG-labeled TYLCV probe staining blue (a and b). Violet color (c) indicates the presence of both viruses. Samples were examined by either combined DIC and epifluorescence microscopy (a1 and c1), DIC microscopy (b1), or epifluorescence microscopy alone to visualize DAPI-stained nuclei (a2, b2, and c2). Bar, 25 μm. Arrows point at the same sites in the corresponding pictures. Note the predominantly TYLCV- or TYLCSV-infected nuclei in neighboring cells (b1) and the presence of both viruses within one nucleus (c1).

Article Snippet: Rehydrated specimens were analyzed with epifluorescence microscopes (Axioskop, Carl Zeiss, Jena, Germany; and Nikon Eclipse E-800).

Techniques: In Situ, Infection, Labeling, Staining, Epifluorescence Microscopy, Microscopy